Quantitative analysis of newly synthesized proteins
نویسندگان
چکیده
منابع مشابه
Getting Newly Synthesized Proteins into Shape
Universitä t Freiburg with the analysis of folding of specific endogenous proteins in mutant strains, these studies have led to signifi-Hermann Herder Str. 7 D-79104 Freiburg cant advances in our understanding of protein folding in the complex cellular milieu. Germany Nascent chains emerging at the peptide exit tunnel of Madison, Wisconsin 53706 the ribosome are awaited by a welcoming committee...
متن کاملGetting Newly Synthesized Proteins Minireview into
Universitä t Freiburg with the analysis of folding of specific endogenous proteins in mutant strains, these studies have led to signifi-Hermann Herder Str. 7 D-79104 Freiburg cant advances in our understanding of protein folding in the complex cellular milieu. Germany Nascent chains emerging at the peptide exit tunnel of Madison, Wisconsin 53706 the ribosome are awaited by a welcoming committee...
متن کاملQuantitative Structure-Cytotoxicity Relationship of Newly Synthesized Piperic Acid Esters.
BACKGROUND/AIM Eleven piperic acid esters were subjected to quantitative structure-activity relationship (QSAR) analysis based on their cytotoxicity and tumor-specificity, in order to find their new biological activities. MATERIALS AND METHODS Cytotoxicity against four human oral squamous cell carcinoma cell lines and three oral normal mesenchymal cells was determined by the 3-(4,5-dimethylth...
متن کاملSelective dye-labeling of newly synthesized proteins in bacterial cells.
We describe fluorescence labeling of newly synthesized proteins in Escherichia coli cells by means of Cu(I)-catalyzed cycloaddition between alkynyl amino acid side chains and the fluorogenic dye 3-azido-7-hydroxycoumarin. The method involves co-translational labeling of proteins by the non-natural amino acids homopropargylglycine (Hpg) or ethynylphenylalanine (Eth) followed by treatment with th...
متن کاملChaperone-assisted folding of newly synthesized proteins in the cytosol.
The way in which a newly synthesized polypeptide chain folds into its unique three-dimensional structure remains one of the fundamental questions in molecular biology. Protein folding in the cell is a problematic process and, in many cases, requires the assistance of a network of molecular chaperones to support productive protein foldingin vivo. During protein biosynthesis, ribosome-associated ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Protocols
سال: 2018
ISSN: 1754-2189,1750-2799
DOI: 10.1038/s41596-018-0012-y